FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9621i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9621i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7919950
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC18750.045.263644
GTCGCCG20300.041.12771245
CGCCGTA21500.038.35227647
TGGTCGC24000.036.3665943
GTGGTCG25050.034.841842
CTCGGTG24050.034.27953738
TCGCCGT26750.031.33990946
CGTGTAG28650.031.06189716
ATCTCGG27100.030.29192736
TGTAGGG30000.029.66411618
AGCGTCG31000.029.59305611
CCGTATC28550.029.48894949
GTCGTGT30450.029.44856614
CGTCGTG30700.029.32017313
GCGTCGT30850.029.17742512
GTGTAGG30450.028.99917217
GATCTCG27900.028.93065835
GGTGGTC30700.028.42900841
GCCGTAT30350.028.19441648
GTAGGGA31900.028.00523819
Produced by FastQC (version 0.11.2)