FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9621c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9621c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300362
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83560.1006703081142726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC13650.034.62706444
GTCGCCG15850.029.6032945
CGCCGTA15900.029.0776347
TGGTCGC17450.027.28256643
CTCGGTG16850.026.4072638
GTGGTCG19200.024.97495342
GTCGTGT20350.024.0656314
AGCGTCG21000.023.8132911
GCGTCGT21050.023.42876612
CGTCGTG21300.022.99172813
CGTGTAG21150.022.66847216
GAGCGTC22300.022.42466710
ATCTCGG19750.022.35427936
CCGTATC21800.021.68476149
TCGCCGT22250.021.55336846
GATCTCG21600.020.76079835
GTGTAGG23950.020.73837917
TGTAGGG24550.020.0912818
GTAGGGA24450.020.03237719
GGTGGTC24700.019.83177241
Produced by FastQC (version 0.11.2)