FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9012i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9012i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7599055
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC20150.046.91446744
CGCCGTA23350.041.0777247
GTCGCCG23400.040.5460145
TGGTCGC24550.039.0667643
CTCGGTG26600.036.44003338
GTGGTCG26900.036.29416742
ATCTCGG27150.035.69972236
GATCTCG28450.034.07024835
TCGCCGT29350.033.03159346
CGTGTAG30450.032.96442416
GTCGTGT31800.031.56249614
CCGTATC30950.031.32769249
AGCGTCG32550.031.1527311
GCCGTAT32100.030.42014748
GCGTCGT33050.030.36855712
GGTGGTC32200.030.31948541
GTGTAGG33150.029.96757717
GTAGGGA33350.029.68403819
TGTAGGG33700.029.4784918
TCGGTGG33550.029.30319839
Produced by FastQC (version 0.11.2)