FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah9012c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah9012c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7731857
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG152610.19737819776025345Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC38700.057.67970744
CGCCGTA40850.054.47677647
GTCGCCG41150.053.65866545
GTGGTCG43100.051.78760542
CTCGGTG42350.051.14971538
TGGTCGC43600.050.87787243
ATCTCGG43800.050.08415636
TCGCCGT44600.049.50761846
GTCGTGT47100.049.4988814
AGCGTCG47300.049.1443911
CGTGTAG47750.048.25506616
GCGTCGT48050.048.2331312
CCGTATC45750.047.74053649
GTAGGGA48500.047.72406819
CGTCGTG49550.046.911913
GTGTAGG49150.046.67061617
GAGCGTC50200.046.5117810
TGTAGGG49550.046.2953618
GATCTCG48000.045.2040535
TCGTGTA52450.044.78069715
Produced by FastQC (version 0.11.2)