FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah8867b-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah8867b-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6985343
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG99110.14188279659280867Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC29700.049.95112644
TGGTCGC31950.046.7553943
CGCCGTA31850.046.0379447
GTCGCCG32650.045.0152545
GTGGTCG34900.043.39515742
TCGCCGT34250.042.9123546
CTCGGTG35200.042.53040338
CCGTATC35550.041.34638249
CGTGTAG38700.040.7328916
GCGTCGT39900.039.9352312
AGCGTCG40600.039.58677311
GTGTAGG39550.039.5088917
GCCGTAT37800.039.43240448
CGTCGTG40350.039.40212213
GTCGTGT40850.038.92207314
ATCTCGG39100.038.55262836
GATCTCG39550.037.76781535
GAGCGTC42900.037.5450710
TGTAGGG42800.036.1060918
GGTGGTC42050.035.9336241
Produced by FastQC (version 0.11.2)