FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah8867a-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah8867a-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7629793
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC18050.039.56723444
CGCCGTA19600.036.0863747
CTCGGTG19800.034.4937238
GTCGCCG21050.034.09210245
TGGTCGC22000.032.617843
GTGGTCG22850.032.00712242
TCGCCGT24550.028.95064746
ATCTCGG24850.027.34404636
GATCTCG25850.026.55520435
CCGTATC26900.026.29565449
CGTGTAG29250.025.94297416
GCCGTAT28300.025.7256948
GTAGGGA30700.024.94301419
GTCGTGT30650.024.86888314
TCTCGGT28650.024.80030437
TGTAGGG30900.024.6699418
AGCGTCG31700.024.26275311
GCGTCGT31600.024.23007212
GGTGGTC30200.023.75991241
GTGTAGG31750.023.683117
Produced by FastQC (version 0.11.2)