FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah7797i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah7797i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6462706
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC7550.017.36347244
CGCCGTA9250.015.29169247
CTCGGTG11200.012.6270538
GTCGCCG11300.012.21175745
TGGTCGC10850.012.08204343
ATCTCGG12400.011.6832536
GTGGTCG11850.011.35340242
TCGCCGT14550.011.14375246
CGTGTAG14400.011.01830116
GCCGTAT14900.010.65202148
CCGTATC14900.010.18912749
GATCTCG15050.010.08499735
GTGTAGG16150.09.610867517
GGTGGTC16350.09.4942641
TCTCGGT17150.09.25158937
GTCGTGT15350.09.21229514
TGTAGGG17250.08.99834918
GTAGGGA16700.08.88160519
CGTCGTG17300.08.57244413
AGCGTCG16900.08.16336211
Produced by FastQC (version 0.11.2)