FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah7797i0h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah7797i0h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740829
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC9000.013.79987744
CGCCGTA12150.011.35829147
GTGGTCG13150.010.75593342
TGGTCGC13600.010.1466443
CTCGGTG12850.09.93222338
ATCTCGG14900.09.49130236
CGTGTAG17250.09.19849216
GTCGCCG14850.08.59588345
GTCGTGT17400.08.523416514
TGTAGGG18650.08.32313918
TCTCGGT18900.08.21272737
GATCTCG17100.08.0690635
TCGCCGT17550.07.86312746
GGTGGTC21550.07.843869741
GTAGGGA20750.07.81327719
TAGGGGG8001.3074168E-77.761226738
CGTCGTG20500.07.73894313
CCGTATC17900.07.71082449
GCGTCGT18400.07.685196412
AGCGTCG18700.07.56200211
Produced by FastQC (version 0.11.2)