FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah7797c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah7797c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7619086
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC10250.018.17634244
GTGGTCG14000.015.27788342
CTCGGTG13000.014.59396338
TGGTCGC13750.014.30145543
CGTGTAG16050.014.18459816
GTCGCCG15800.013.75688345
CGCCGTA13700.013.59926647
GCGTCGT16350.013.28844112
AGCGTCG16400.013.03789911
GTGTAGG17450.012.84890417
GTCGTGT18000.012.45409214
GGTGGTC18750.012.32703941
GAGCGTC18350.011.8401110
GTAGGGA20600.011.3866419
GATCGGA20750.011.1467962
CCGTATC16750.011.12400549
TGTAGGG19900.011.09381118
CGTCGTG20550.011.07593813
TCGTGTA22250.010.85080715
ATCTCGG17650.010.7487436
Produced by FastQC (version 0.11.2)