FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah7797c0htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah7797c0htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649995
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC9800.020.77121244
TGGTCGC14500.014.51334943
CGCCGTA13700.014.35473747
CTCGGTG13500.014.30901738
CGTGTAG16100.013.28346316
GTGGTCG14700.013.1421542
AGCGTCG17050.012.33916211
GTCGCCG17000.012.17701245
GCGTCGT17500.011.8247212
GTCGTGT16800.011.70202114
TCGCCGT19350.011.23292946
ATCTCGG17800.010.85165336
GCCGTAT21750.010.62904348
GATCTCG19850.010.42686735
GTGTAGG20500.010.09590817
GAGCGTC23250.010.08703810
GTAGGGA20650.010.02257219
TCTCGGT22400.010.0092937
GGTGGTC20850.09.92735141
TGTAGGG21000.09.69143918
Produced by FastQC (version 0.11.2)