FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah6408i-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah6408i-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414883
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG186510.29074575483294085Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83140.12960485795298216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC44800.060.9155144
GTCGCCG46950.058.63945845
CGCCGTA47900.057.47780247
GTGGTCG49550.055.49115442
CTCGGTG48550.054.7095538
CCGTATC51950.053.20024549
TGGTCGC51250.053.11277843
GATCTCG51000.052.82701535
ATCTCGG51600.052.4092236
GCCGTAT53150.052.3873948
TCGCCGT52600.052.2751446
CGTGTAG56100.051.7714816
GTCGTGT58350.051.4271714
GTGTAGG57050.051.0911617
GGTGGTC53650.050.86303741
AGCGTCG59100.050.65821511
GTAGGGA58100.050.4642919
GCGTCGT59400.050.4600412
TGTAGGG58000.050.37327218
CGTATCA57400.049.23100350
Produced by FastQC (version 0.11.2)