FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah6408a-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah6408a-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8749059
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG157500.18001935979629352Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC41050.056.14206344
GTCGCCG43150.053.3298245
CGCCGTA43800.052.6962347
CTCGGTG44450.050.2111838
TGGTCGC47150.048.8770443
ATCTCGG46850.048.44727336
GTGGTCG47450.048.2766442
TCGCCGT49800.046.4855846
GATCTCG49150.045.41022535
CCGTATC51600.044.66723649
GCCGTAT53650.044.24646448
TCTCGGT52800.042.85564837
GTAGGGA59300.040.8359719
GGTGGTC56150.040.61107641
CGTGTAG59850.040.4595516
TCGGTGG57300.040.45620339
TGTAGGG60400.040.0351618
CGTATCA58800.039.60830750
GTGTAGG61350.039.58344717
GTCGTGT63550.038.2621614
Produced by FastQC (version 0.11.2)