FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah5184i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah5184i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9007994
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC10850.022.89475344
CGCCGTA14950.017.53942347
GTCGCCG16800.015.81292645
CTCGGTG15950.015.78697438
TGGTCGC17350.015.11221143
GTGGTCG18150.014.25555442
ATCTCGG20100.013.55652836
CGTGTAG22000.013.48350616
GTCGTGT22550.013.1531814
CCGTATC22500.012.8818549
GATCTCG21900.012.60119135
TCGCCGT22300.012.22230246
GTGTAGG24500.012.10770617
GCGTCGT24200.011.82836412
GCCGTAT25250.011.75192248
AGCGTCG24550.011.65992611
TGTAGGG25150.011.65789318
GTAGGGA24150.011.56923419
TCTCGGT25150.011.24581337
GGTGGTC25350.011.02267941
Produced by FastQC (version 0.11.2)