FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah5184c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah5184c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309749
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC20950.041.00552444
CTCGGTG21700.039.1040938
AGCGTCG26650.035.97733711
GTCGCCG25200.035.86970545
GTGGTCG24750.035.68428442
CGCCGTA25300.035.5927847
TGGTCGC24650.035.40941643
GCGTCGT28000.034.11907612
CGTGTAG28150.033.5747716
GTCGTGT28500.033.4008514
TCGCCGT27550.033.06049746
GTGTAGG28450.032.85717
CCGTATC28600.032.4534549
GAGCGTC29500.032.2681810
TGTAGGG29750.031.76906818
ATCTCGG27250.031.64610936
GGTGGTC28350.031.51715541
GCCGTAT29900.031.38863248
GATCTCG27800.030.52557635
GTAGGGA31400.030.3191719
Produced by FastQC (version 0.11.2)