FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah3988i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah3988i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8125105
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC9750.021.23115244
CGCCGTA12600.015.8816347
GTCGCCG13000.015.65807145
GTGGTCG14650.015.30621842
TGGTCGC14600.014.41357843
CTCGGTG14900.014.12180738
ATCTCGG15850.012.83989336
AGCGTCG18300.012.62733311
GCGTCGT18900.012.04383212
GTCGTGT19700.011.55495514
CGTGTAG19350.011.23075616
GTAGGGA21200.011.06432919
GAGCGTC21200.011.06262710
TGTAGGG21950.010.84349418
GATCTCG19100.010.83635735
TCGCCGT18400.010.68769346
CCGTATC18800.010.6451249
GTGTAGG22100.010.30151517
CGTCGTG23150.010.13053613
TCTCGGT21850.09.94528237
Produced by FastQC (version 0.11.2)