FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah3988c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah3988c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8858986
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC16100.042.2161744
GTCGCCG19450.034.94500745
CGCCGTA20000.034.15710447
GTGGTCG21300.032.3925242
TGGTCGC21500.032.09264443
CTCGGTG21700.030.52033238
AGCGTCG25000.028.6939411
ATCTCGG24300.027.96264636
CGTGTAG25400.027.56659316
GCGTCGT26050.027.40451212
GTCGTGT26350.026.96223614
GTGTAGG26850.026.59174517
TCGCCGT25550.026.19671646
CGTCGTG26800.026.12226913
GATCTCG25650.025.95523835
TGTAGGG27900.025.83838318
GAGCGTC28850.024.9845910
CCGTATC26850.024.6745649
GGTGGTC28350.024.45809641
GTAGGGA29350.024.20930519
Produced by FastQC (version 0.11.2)