FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah119i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah119i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032030
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC8800.019.6031844
GTGGTCG12200.015.26978742
GTCGCCG10800.015.01458545
CGCCGTA10950.014.80922147
TGGTCGC11650.014.80681543
CTCGGTG12650.013.36111838
ATCTCGG14500.011.65592636
TCGCCGT15950.010.81554846
CGTGTAG16350.010.75882716
GATCTCG15100.010.7367735
GCGTCGT17150.010.457108512
GCCGTAT17250.010.40159148
GTAGGGA17950.010.18427519
GTCGTGT16950.010.17362514
CCGTATC17000.010.14875649
CGTCGTG18500.09.88023513
TGTAGGG18200.09.85493518
GTGTAGG18550.09.85472417
AGCGTCG17750.09.71502711
TCGGTGG19750.09.43146939