FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n02_ah119c3htop2-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n02_ah119c3htop2-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6987582
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG108920.15587652495527066Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC30100.052.84137744
TGGTCGC33250.048.14423443
GTGGTCG33350.047.89436342
GTCGCCG33500.047.6840145
CTCGGTG33700.047.08444638
CGCCGTA34800.046.2011347
AGCGTCG37900.045.04505511
GCGTCGT38350.044.60642612
GTCGTGT39450.043.62523714
CGTGTAG39100.043.2265116
GAGCGTC39900.042.96064810
GTGTAGG39400.042.8101417
TGTAGGG40000.042.34075518
CCGTATC38200.041.81982449
TCGCCGT38050.041.8003346
CGTCGTG41200.041.68821313
GGTGGTC38700.041.18239641
GCCGTAT39750.040.9700348
TCGGTGG39850.040.59816739
GATCTCG38600.040.48359735
Produced by FastQC (version 0.11.2)