FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9847i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9847i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7649881
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG27650.035.30386729
CACGGCT27850.034.92740231
AGTCACG29650.033.1540828
TCACGGC30000.032.42342430
CGGCTAC29850.032.24095533
GCACACG32050.031.31650211
GCCGTCT31200.030.86447749
ACGGCTA32200.029.88757532
ACGTCTG34600.029.50788715
ACACGTC35000.029.46630313
TGCCGTC33300.029.1234648
CACACGT34700.029.02448512
CATCTCG29650.028.96979339
CACGTCT35650.028.73554214
GTATGCC35000.027.9948345
CGTCTGA37350.027.6123316
GGCTACA34450.027.2350234
TATGCCG36300.026.52172746
CGTATGC38300.025.76273244
TCCAGTC37800.025.73220425
Produced by FastQC (version 0.11.2)