FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9847c3hmyc-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9847c3hmyc-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6675544
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG86450.12950255439856287TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC25100.042.87592729
GCACACG28350.038.93375811
TCACGCC28650.037.56320630
AGTCACG29350.037.1371428
ACACGTC29800.036.69263513
GCCGTCT28400.035.97214549
ACGTCTG31000.035.71708715
CACACGT30900.035.60888312
CACGTCT31350.035.09827814
CACGCCA31400.034.49390831
TGCCGTC30900.033.72790548
GTATGCC32400.033.5425345
CGTCTGA33600.032.9535116
GATCGGA32900.032.8227841
AGCACAC34150.032.72530710
TCCAGTC34350.032.3336925
GAACTCC34600.032.09982721
TATCTCG32750.031.70446239
CGTATGC34350.031.53769544
CAGTCAC34850.031.37491827
Produced by FastQC (version 0.11.2)