FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9767i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9767i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7247956
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG24950.034.8370328
GTCACGA25950.032.96426429
CGATCAG26300.032.5262533
GCACACG27950.030.97397811
GCCGTCT27800.030.16937349
CACGATC29200.029.29549831
ACGTCTG29800.029.28419715
TGCCGTC29300.028.9766648
CACGTCT30550.028.33946214
ACACGTC31050.027.99458313
CACACGT30950.027.86040712
CGTCTGA30500.027.82065216
GTATGCC30150.027.80807545
TATGCCG31300.027.4517446
GATCTCG25750.026.92674439
CAGTCAC32500.026.63780627
CGTATGC31750.026.62387844
TCCAGTC32950.026.16933425
GAACTCC34100.025.28644421
ATGCCGT34800.024.59468747
Produced by FastQC (version 0.11.2)