FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9767c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9767c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6552917
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA29450.033.37951717
CACGTCT30350.032.6169715
ACGTCTG30300.032.44312316
GCACACG30550.031.95133612
ACACGTC30450.031.9434814
GTATGCC29200.031.9075646
ACTGACC29150.031.59557733
GATCGGA32050.031.1068822
TCCAGTC32100.030.19384426
CACACGT32900.029.98406813
CGTATGC31700.029.82183545
GCCGTCT29700.029.5186250
CAGTCAC32700.029.42887128
AGATCGG33550.029.4092121
TGCCGTC31000.029.28125249
GAACTCC32900.029.2499722
CCAGTCA33100.028.96902827
TCGTATG34400.027.1794544
CTGACCA34200.026.9301534
AGCACAC36450.026.77951611
Produced by FastQC (version 0.11.2)