FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9624i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9624i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8626279
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG26250.017.87034829
GCACACG29950.015.89225412
GTCACGT29500.015.78465730
ACGTCTG31650.015.47517216
GTTTCGG29750.015.42057835
CGTTTCG30500.015.15430234
ACACGTC32450.014.98727814
CACACGT32500.014.8580913
CGTCTGA33250.014.83415217
TCCAGTC33400.014.14742326
CACGTCT35050.013.58030115
CAGTCAC35050.013.48150328
CCAGTCA37600.012.842319527
GAACTCC38350.012.68110322
GCCGTCT22100.011.87299552
AGCACAC40450.011.85221511
GTATGCC27400.011.83940448
TGCCGTC25750.011.53061951
ACTCCAG42100.011.38763124
GTCTGAA44500.011.08394618
Produced by FastQC (version 0.11.2)