FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9624c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9624c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7155206
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22550.021.26127211
GCCGTCT22950.018.94868349
ACACGTC25700.018.6555813
ACGTCTG25000.018.62605315
CACACGT26200.018.43069312
GTATGCC25550.018.22981545
TGCCGTC24250.018.21574448
CGTCTGA27500.017.93632316
CACGTCT26600.017.89470514
CGTATGC27250.016.96557444
CAGTCAC29150.016.80226127
TATGCCG27500.016.18631646
TCCAGTC29850.016.17713725
CCAGTCA30400.015.88445926
GATCGGA29300.015.7784521
AGCACAC30400.015.77121810
AGATCGG27150.014.9947571
GAGCACA33200.014.7524859
GTCACCT32650.014.68448329
AGTCACC33850.014.57109828
Produced by FastQC (version 0.11.2)