FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9621i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9621i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7919950
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGGC28150.030.63448133
AGTCACG29800.029.16904429
CACGTGG30000.028.7451732
GCACACG33650.027.36811812
TGCCGTC31400.026.38515751
ACGTCTG35500.026.13687516
GTCACGT33100.025.84411230
GCCGTCT30950.025.76495652
CACACGT35800.025.72498913
CGTCTGA35850.025.49668317
CGTGGCC34700.025.44833834
ACACGTC36650.025.0344114
TCACGTG34650.024.98714431
GTATGCC33900.024.94125448
CAGTCAC36200.024.39230728
CACGTCT38300.024.13602315
GGCCTTA35600.023.7398937
TCCAGTC37650.023.3614326
CGTATGC37000.022.94369147
TATCTCG32300.022.85735542
Produced by FastQC (version 0.11.2)