FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9621c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9621c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300362
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28650.022.87415911
CACACGT30950.021.9545412
ACACGTC30600.021.52972813
ACGTCTG32500.020.16493615
CACGTCT33700.019.44689814
GCCGTCT27700.019.43834749
TGCCGTC29700.018.94046648
CGTCTGA34850.018.90426616
CATCTCG28700.018.87062339
AGCACAC35700.018.6469510
GTATGCC30900.018.4232945
TCCAGTC37500.018.02783425
GATCGGA35850.017.8029881
CAGTCAC37400.017.79936227
CGTATGC32800.017.35566944
CCAGTCA38450.017.13388326
AGTCACC39150.017.00363228
GAGCACA39650.016.963199
TATGCCG35400.016.66803246
AGAGCAC41600.016.1680418
Produced by FastQC (version 0.11.2)