FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9012i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9012i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7599055
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA124510.16384932073790756TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA95770.1260288285846069TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT51400.035.7021331
CGAGTGG52250.034.92327533
ACGAGTG53500.034.1717932
AGTCACG54400.034.11169428
GTCACGA55100.033.5544629
TGCCGTC56350.032.52803450
GCCGTCT55000.032.3882251
TCACGAG57100.032.3791730
GTATGCC57050.031.69681547
TATCTCG55800.031.65326541
ACGTCTG59100.031.633215
GCACACG58700.031.55349511
CGTATGC57800.031.46277646
CGTCTGA59750.031.4045316
ACACGTC60650.030.71122613
TATGCCG60350.030.47884648
CACGTCT61600.030.34938214
CTCGTAT59600.030.10132244
CACACGT62000.029.92966812
CAGTCAC61600.029.90043827
Produced by FastQC (version 0.11.2)