FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah9012c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah9012c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7731857
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA181280.23445855245382835TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA128520.1662213876950906TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG56450.042.4758531
GCACACG76100.031.09338812
ACACGTC78650.030.17356914
ACGTCTG79050.030.02108216
CACACGT79700.029.8193313
GCCGTCT69050.029.79572352
CGTCTGA80300.029.63966817
CACGTCT80200.029.50439615
GTATGCC75750.029.11158848
TATGCCG76300.029.08529349
GATCGGA82500.029.0189592
CGTATGC76500.029.00531447
TGCCGTC74100.028.92900551
GTTCCGT81050.028.64264136
AGTTCCG83100.028.43417435
CAGTCAC82550.028.413428
CCGTATC73300.028.14247539
AGCACAC85250.028.03913511
GTCACAG84450.027.97908830
TATCTCG70750.027.94003742
Produced by FastQC (version 0.11.2)