FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah8867b-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah8867b-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6985343
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA144820.207319812355671TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA128740.1843001839709231TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT65250.032.32807551
AGATCGG50200.032.2312551
AGTCACG69450.031.33782428
TGCCGTC69200.030.72714450
ACACGTC72700.030.64942213
ACGTCTG72850.030.39692515
CACGTCT73050.030.313714
GCACACG72950.030.11798111
GATCTCG58050.029.94972841
CGATCTC58100.029.8639440
CGTATGC71750.029.81749546
GTATGCC71750.029.67576847
CGTCTGA74650.029.61798316
GAACTCC73350.029.57734121
TATGCCG71500.029.53968648
CACACGT75400.029.23083712
CAGTCAC75500.029.05506727
GTCACGT76400.028.44232729
CTCGTAT67850.028.37277244
ATGCCGT75350.028.30759849
Produced by FastQC (version 0.11.2)