FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah8867a-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah8867a-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7629793
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG34200.031.16510832
CGTCCGC36400.029.66074833
CACGTCC38750.027.6837531
GTCCGCA39150.027.31297734
CCGCACA37850.027.24950836
AGTCACG40000.027.24872828
GCCGTCT40400.025.3789151
CATCTCG36350.025.14815541
GTATGCC41750.024.87923447
GTCACGT43750.024.83509829
TCCGCAC43250.024.80417335
TGCCGTC42600.024.55158850
TCACGTC44700.024.30728330
ACGTCTG45900.024.19752315
CGTATGC44350.023.73053746
CGTCTGA46650.023.51289216
GCACACG46850.023.4853711
ACACGTC46450.023.46546213
CACGTCT46500.023.4402314
TATGCCG44850.023.39165348
Produced by FastQC (version 0.11.2)