FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah7797i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah7797i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6462706
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC15250.021.03601333
ACTTAGG16650.019.05989632
GCACACG18850.017.93183511
TGCCGTC19050.017.21025748
GCCGTCT18200.017.06737949
CACACGT20100.016.81667112
ACACGTC20000.016.72972113
CATCTCG17500.016.16440639
ACGTCTG20950.015.64179315
CGTCTGA22100.015.60826816
CACGTCT21450.015.43799614
GTATGCC21150.015.1709845
CAGTCAC22550.014.83720127
TTAGGCA22750.014.70756134
CGTATGC21550.014.56873144
ATGCCGT23650.014.44594547
CCAGTCA24550.014.19035726
TCCAGTC24300.013.91051725
CACTTAG24050.013.62559631
TATGCCG24350.013.32121846
Produced by FastQC (version 0.11.2)