FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah7797i0h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah7797i0h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740829
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24450.021.30132511
GCCGTCT23950.021.03982449
GTATGCC25500.020.4302845
CGATCTC21650.020.39453938
TGCCGTC25950.020.0814548
GATCTCG22550.019.88702839
CACGATC22500.019.47051436
ACACGTC27050.018.99996413
CAGTCAC29000.018.91070427
CACACGT27800.018.8586312
ACGTCTG27150.018.80293515
CGTCTGA28000.018.60182216
CACGTCT27450.018.59743914
CATCACG28000.018.4788733
ATGCCGT28800.018.09352147
TCCAGTC31450.017.4375325
CGTATGC29550.017.39645644
CCAGTCA30850.017.32946226
GTCACAT32900.016.87944629
TATGCCG30550.016.82973346
Produced by FastQC (version 0.11.2)