FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah7797c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah7797c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7619086
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19750.016.24155411
ACGTCTG20100.015.44455115
ACACGTC21400.014.98997313
GCCGTCT20950.014.00674651
CACGTCT22350.013.8897314
CGTCTGA23550.013.3284416
CACACGT24550.013.06610112
GTATGCC24000.012.65343347
TGCCGTC23400.012.5389150
GATCGGA23950.012.2442051
TCCAGTC24900.012.18959825
GAACTCC25250.012.15715121
TATCTCG23600.012.13214741
AGCACAC27400.011.45527210
CGTATGC28450.010.673408546
AGATCGG22000.010.66358951
ATCTCGT26100.010.5742542
CCAGTCA29250.010.49470326
CAGTCAC28650.010.47371327
GAGCACA29700.010.4518939
Produced by FastQC (version 0.11.2)