FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah7797c0htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah7797c0htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649995
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22350.025.77263611
ACACGTC25350.022.58773213
GTCACAC26050.021.9806429
TGCCGTC25500.021.92747350
CACACGT26300.021.9018412
ACGTCTG26600.021.78563315
GCCGTCT26000.021.50827851
CACGTCT26800.021.36563514
GTATGCC27750.020.76726547
CGTATGC27750.020.51641846
CGTCTGA29450.019.56021916
TATCTCG28150.019.4848241
CAGTCAC30200.019.41654227
TCCAGTC32900.017.82308825
GATCGGA31050.017.6670251
AGCACAC33300.017.5051110
CACACTG34000.017.14540731
TATGCCG33950.016.7716248
CCAGTCA34150.016.7666926
ATGCCGT33550.016.6647449
Produced by FastQC (version 0.11.2)