FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah6408i-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah6408i-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414883
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA183140.28549234646992TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG64000.045.2746636
AGATCGG68400.044.8894161
GTCCCGA57800.044.87892537
ACCCGTC68350.041.63589533
CACCCGT69400.041.45264432
TCACCCG69100.041.3830231
GCACACG75500.040.2492412
ACGTCTG75450.040.04889716
TCCCGAT55450.039.9385938
GTATGCC70650.039.9063348
CACGTCT75950.039.8760815
CCCGTCC73050.039.71259334
TGCCGTC69550.039.70606651
GCCGTCT67350.039.56889352
ACACGTC76700.039.4414914
CCCGATC49600.039.294939
ATGCCGT70500.039.1156550
CACACGT77700.038.756613
GAACTCC77700.038.665122
CCGTCCC75050.038.6086335
Produced by FastQC (version 0.11.2)