FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah6408a-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah6408a-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8749059
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA102330.11696114976479184TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGT78150.032.0444332
TCACATG79300.031.53622831
CAGTCAC79050.031.50397728
GTATGCC58250.031.39847648
GCACACG78650.031.1817812
GAACTCC79500.031.10835622
ACACGTC80000.031.08746114
CGTCTGA79950.031.06340417
CACACGT80150.030.72802413
GTCACAT81450.030.66090430
TCCAGTC80750.030.54172526
ACGTCTG81600.030.52017216
CACGTCT81500.030.51529915
AGTCACA81500.030.38815329
CCAGTCA81750.030.25251227
GCCGTCT49900.029.67747552
TATGCCG61100.029.65540349
CGTATGC60800.029.5126747
TGCCGTC57100.029.44154451
ATGTCAG86450.028.76918835
Produced by FastQC (version 0.11.2)