FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah5184i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah5184i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9007994
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28300.017.06279811
GTCACAC28550.016.55169329
ACACGTC30700.015.8420413
ACGTCTG30950.015.60262915
CACGTCT31700.015.45110214
CACACGT31150.015.39103412
CGTCTGA32250.014.8667316
GTATGCC26800.014.67571545
GCCGTCT23600.014.62475149
TGCCGTC25900.013.99093148
TCCAGTC33850.013.75581125
CGTATGC28450.013.21808844
AGCACAC36850.013.19760510
CAGTCAC36100.013.1851627
GAACTCC36750.012.76417321
AGTCACA39100.012.6145128
ATGCCGT31400.012.41892947
CCAGTCA40050.012.22916726
GAGCACA40400.012.2085979
TATGCCG32300.011.85822346
Produced by FastQC (version 0.11.2)