FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah5184c3htop2-spikein-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah5184c3htop2-spikein-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7309749
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG33250.028.9492931
GCACACG40650.024.09839612
CCGATGT41250.023.49766234
ACACGTC42200.023.13216214
CACGTCT42150.023.07776515
ACGTCTG43400.022.81047816
TCCAGTC43800.022.60154726
CACACGT43600.022.54761513
CGTCTGA44050.022.39527717
GAACTCC43500.022.36079422
CAGTCAC45500.021.7572428
GATCGGA44950.021.4889472
CCAGTCA46500.021.43755527
CACCGAT46150.021.30175632
GCCGTCT37800.021.27389749
GTCACCG45400.021.27362330
GTATGCC40200.021.19821445
AGTCACC47600.021.01504329
ACCGATG47150.020.7768133
CGATGTA46250.020.73405335
Produced by FastQC (version 0.11.2)