FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah3988i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah3988i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8125105
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG17800.019.76599133
GCCGTCT21200.015.79605451
CGTACGT22400.015.24493434
GCACACG23100.015.0808711
TGCCGTC24350.014.31718350
GTATGCC24400.014.14281147
ACACGTC25500.014.06771413
TATGCCG25950.013.69758948
CACACGT26450.013.5620312
ACGTCTG26000.013.53185215
CACGTCT26400.013.19616814
CGTATGC26700.013.18195146
ACCGTAC26850.013.10362932
CGTCTGA27000.013.03075216
GTACGTA26950.012.79942135
CAGTCAC28500.012.70753927
GTCACCG28000.012.19557329
GATCGGA29400.011.7343191
ATGCCGT29850.011.56254449
AGCACAC30300.011.49736810
Produced by FastQC (version 0.11.2)