FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah3988c3hflag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah3988c3hflag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8858986
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28600.025.20587512
ACGTCTG32100.022.99522216
ACACGTC32350.022.17777314
CACGTCT34000.022.11599315
GCCGTCT30250.021.68376752
TGCCGTC31300.021.61752751
CACACGT34100.021.24192213
CGTCTGA36550.019.91230417
CGTATGC35750.019.78703747
AGATCGG36200.019.72871
GTCACAG37900.019.47660430
AGCACAC37850.019.41032211
CAGTCAC38400.019.13231328
GTATGCC35950.019.10201648
CACAGTC37750.019.09723332
TCCAGTC38950.018.77348926
GATCGGA38700.018.541822
CCAGTCA40300.018.14460227
TATGCCG37700.018.12552349
AGTCACA42000.018.06808529
Produced by FastQC (version 0.11.2)