FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah119i3h-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah119i3h-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032030
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18700.014.01803711
ACGTCTG20000.013.45197315
CACACGT20150.012.8381212
TGCCGTC17850.012.37356448
ACACGTC20500.012.28260313
CGTCTGA21800.012.02481716
CACTAGC23200.011.59677731
CACGTCT22000.011.44515214
GCCGTCT18100.011.44137549
GTATGCC21850.011.36853145
CAGTCAC23850.011.28031927
TATGCCG21850.011.2123946
TATCTCG20550.011.07882439
TCCAGTC25000.011.03735925
CGTATGC21650.010.99540944
GAACTCC25850.010.54099821
CCAGTCA24600.010.51577726
AGCACAC24900.010.1121110
GATCGGA23900.010.1049881
GAGCACA25950.010.1017029
Produced by FastQC (version 0.11.2)