FastQCFastQC Report
Wed 28 Mar 2018
HW5G7AFXX_n01_ah119c3htop2-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW5G7AFXX_n01_ah119c3htop2-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6987582
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG76180.10902197641473116TruSeq Adapter, Index 5 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG70890.10145140336099098TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG47150.037.1690371
ACGTCTG58150.029.71859416
CACGTCT59150.029.2744815
GCACACG59050.029.26480312
CGTCTGA59550.029.19348117
ACACGTC61150.028.31701514
GATCGGA61650.028.3123742
TCCAGTC60700.027.90105226
TGCCGTC54400.027.53485949
GTCACAC61300.027.51601430
GAACTCC62500.027.48342722
GTATGCC55500.027.48012746
CAGTCAC63200.026.7973728
CACACGT64050.026.76582113
CACACAG63500.026.5628932
CCAGTCA63800.026.4372327
AGTCACA63950.026.26772329
CGTATGC57500.026.04011745
GCCGTCT53500.025.87158650
GAGCACA66050.025.79794110
Produced by FastQC (version 0.11.2)