FastQCFastQC Report
Fri 18 Oct 2019
HW3KNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3KNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681063
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTGCAACTG1393663.7860259387030326No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC1224073.325316627289454No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGTACTGGT989252.6874030680811494No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACGTACTGGT902992.4530685837216044No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGGAACTGG717331.948703404424211No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGGAACTGG681101.850280747707931No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC671131.823196180016479No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA653851.7762532181600805No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT583181.584270630521673No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA577231.5681068213176466No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC546401.484353840181491No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT530751.441838947065019No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAA502111.3640353343585807No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG487041.3230960730636776No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAA482841.3116863253902473No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC479301.302069538065499No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC458031.2442873159193417No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGATCCCCTAC442561.202261411988874No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCA424671.153661320113239No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGAACCCCTAC405221.1008233219589016No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA401521.0907718775799273No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG398021.0812637545187356No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG385671.047713663145673No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGG384861.045513211808654No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTCCAACTG357010.9698557183074562No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCA348220.9459767463909202No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATCCCCTAC334820.9095742180995001No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACTGG314450.8542369418833635No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACC299770.8143571571581361No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGATCCCCTAC265290.720688561972452No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA239750.6513064296916407No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACC232930.6327791727552612No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCGGTACCAA221280.601130705994437No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAA217200.5900469511116762No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCGCTACCA215450.5852928895810803No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT206210.5601914446995337No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTTGCAACTG199330.5415011913678196No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACGGCTACCA182080.49463972770908843No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG178350.4845067851324468No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG177510.4822248355977608No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCAACTGGT162420.4412312421710794No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGAACCCCTAC154020.418411746824219No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC146830.3988793454499421No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACGGCTACCA140460.3815745614785729No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAA139610.37926544587799776No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTAGGAACTGG130650.35492465084134667No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA127360.3459870151638263No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGGTACCAA124800.3390325022962117No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACGCCTACC123800.33631589570729975No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACGGCTACCA119520.3246888195067566No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTG118720.32251553423562707No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGG117710.319771761580826No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTTGCAACTG116580.31670199613535543No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAAGGCCTACC112970.3068950463493833No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTAGGAACTGG106010.2879874644905561No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCA101690.2762517240264565No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACGGCTACCA94030.25544251755539094No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGGT83690.22735280542604133No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG78220.21249296738469295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77740.2111889962220152No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAA75950.20632627042786283No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGG71490.1942102050413155No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGGGAACCGGTACCAA63400.1722328577370178No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCAA59480.16158375990848298No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCGCTACCAA59460.16152942777670473No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATGCCCTAC58820.15979079955980108No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCGTACCAA54830.14895153927004237No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAA52690.1431380011697708No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAAGGCCTACC51990.14123637655753243No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGATGCCCTAC43250.11749323497044195No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTACGAACTGG40270.10939774733548435No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGACCGAACCCCTACC39940.1085012671611434No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAAGGCCTACGTACTGGT39500.10730596026202215No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTG39220.10654531041712681No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG39200.10649097828534855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG360400.0140.879175
CGATTCC327650.0140.699923
TCGCATC367200.0140.674227
ATACAGG390050.0140.515757
TAACGCA362800.0140.501487
CAATGGT422100.0140.474234
ACTGTAT399750.0140.423223
CTGTATC399800.0140.387914
GATACAG390500.0140.367176
GTATCTC403000.0140.295156
ACAATGG422900.0140.107743
ATTCCTT329000.0140.079425
ACGATCG336950.0140.034941
GATTCCT329350.0140.01684
ATGGTCT423650.0139.943516
ACGCATT364100.0139.882839
CGCATCT370550.0139.57498
AATGGTC425350.0139.517765
TATCTCT405650.0139.452917
AACAATG427650.0139.298752
Produced by FastQC (version 0.11.5)