FastQCFastQC Report
Fri 18 Oct 2019
HW3KNAFXY_n01_PCR2_R6_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3KNAFXY_n01_PCR2_R6_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65729270
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTGCAACTG47693257.2560139493409865No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC30717444.673327423231689No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGTACTGGT29727554.5227263287725545No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACGTACTGGT26357814.010056706852214No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGGAACTGG23319943.547877528534852No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGGAACTGG22228683.3818540811422366No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC17923072.7268019255348492No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA14338872.1815045260657846No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT14253752.168554435489699No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT13632092.073975566745226No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAA13432952.0436785620774427No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC13043361.9844066425809992No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC12941191.9688625782699243No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG12939091.9685430859037383No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGATCCCCTAC12772681.9432255979718018No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAA12504851.9024781501452852No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG12036441.8312146171713148No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA11948741.8178720074024861No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTCCAACTG11411771.7361778093686422No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG10988751.6718198756809564No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC10907461.6594524783251055No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGG9788811.4892619376420886No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATCCCCTAC9756041.4842763353373618No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGAACCCCTAC9684421.473380124258188No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA9577961.4571833826847613No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACTGG9470011.4407599536705642No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCA8737301.3292860243237146No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCA8603511.3089313178132056No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGATCCCCTAC7995961.216499133491061No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTTGCAACTG7020251.0680553731997937No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACC6312760.9604183950316197No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA6267370.9535127957453353No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT5987060.9108666504283403No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG5921980.900965429861004No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACC5527180.8409008650179745No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAA5420440.8246615244623895No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG5246150.7981451794611442No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCGCTACCA5110730.7775424860187858No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCGGTACCAA4700100.7150695572915993No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCAACTGGT4028330.612866992133033No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC4014390.6107461713784438No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACGGCTACCA3733990.5680863335314693No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGG3410630.5188905947076546No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTG3399610.5172140204812863No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGAACCCCTAC3364630.511892190495954No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACGCCTACC3219360.48979092571695987No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGGTACCAA3218000.4895840163750487No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACGGCTACCA3091730.4703733968139308No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA2966700.45135142988808485No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTTGCAACTG2920420.44431042669422616No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTAGGAACTGG2836460.43153681761565277No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACGGCTACCA2766130.4208368661328507No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTAGGAACTGG2713300.4127993510349347No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAA2612130.3974074259458534No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACGGCTACCA2473820.37636505015193383No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGG2216650.33723940643186817No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGGT2182880.33210166490514803No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCA1850730.28156862232001056No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAA1850690.28156253675113085No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAAGGCCTACC1699580.25857277891569463No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCAA1675980.2549822932766483No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCGCTACCAA1649780.25099624566041884No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCGTACCAA1405330.2138058128441104No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGGGAACCGGTACCAA1281860.19502118310457428No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAA1131200.17209988791903516No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGACCGAACCCCTACC1099970.1673485800161785No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCTCCGATCCCCTAC1025140.15596400203440566No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG908880.1382762960854426No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCGTACCAA864410.13151066488339214No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACGCCTACCA826060.1256761257199418No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG771040.11730542572586003No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTACGAACTGG748410.11386251513214737No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAAGGCCTACGTACTGGT710530.10809948140303399No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAAGGCCTACC665410.10123495970668775No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTG658720.10021714831155132No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATGCCCTAC657940.10009847971839639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG7675300.0144.7575
TACTGTA11069900.0144.731112
ATAACGC7694250.0144.689796
TCGCATC10892950.0144.630367
ACGATCG8398900.0144.621491
ATACTGT11084450.0144.462551
TAACAAT10767500.0144.445661
GGTCGCA10871150.0144.417025
CGATTCC7558200.0144.414383
TAACGCA7714250.0144.321477
ATACAGG8669600.0144.273217
TCGATTC7571850.0144.233642
GATTCCT7573350.0144.212624
GTCGCAT10906400.0144.192926
GATACAG8685350.0144.18086
TACAGGT8677550.0144.130178
CGATAAC7717600.0144.085744
TGTATCT11137600.0143.999045
TAGGTAA8504700.0143.965039
CTGTATC11131050.0143.932654
Produced by FastQC (version 0.11.5)