FastQCFastQC Report
Fri 18 Oct 2019
HW3KNAFXY_n01_PCR2_R5_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3KNAFXY_n01_PCR2_R5_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34715923
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC21193666.10488161297051No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTGCAACTG14519874.1824813357259725No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGTACTGGT14034244.042594517795192No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACGTACTGGT12372403.5638977537771357No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC12247053.527790403268264No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA11221693.232433140262467No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAA9494542.734923683290806No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAA9382112.7025379679520545No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGGAACTGG8929832.5722576928172125No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA8584162.4726866688810203No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGGAACTGG8471482.440228940477832No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC7909502.278349332667894No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC7787532.243215598790215No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGATCCCCTAC7618612.194557811411207No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC7528382.16856685619449No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA6806731.9606939443897256No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT6749171.9441136564336774No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT6472151.8643174199919732No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCA6205571.7875284491211711No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCA6064741.7469620496623408No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATCCCCTAC5846181.684005348208659No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGAACCCCTAC5830161.6793907510395156No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4919361.4170327546814756No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGATCCCCTAC4741891.3659121204987117No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA4390051.2645638141322069No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACC4331751.247770367505424No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG4202551.2105540158042176No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAA3977231.1456500810881507No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACC3745951.0790293549158982No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGG3702211.0664299491619451No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG3658031.0537038004145822No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACTGG3578761.030869897942797No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCGCTACCA3544471.0209925860245743No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTCCAACTG3426190.9869217649779901No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCGGTACCAA3002580.8648999480728194No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT2816900.8114144048539339No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACGGCTACCA2554690.7358842223494965No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGAACCCCTAC2419630.6969798844178794No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACGCCTACC2193580.6318656715536557No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGGTACCAA2171250.6254334646381143No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTTGCAACTG2139320.61623595604818No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACGGCTACCA2113270.6087321947338113No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCA2056220.5922988134292152No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGAACCCCTAC2013580.5800162651587861No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACGGCTACCA1904060.5484687818900854No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCAACTGGT1891300.5447932350812047No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAA1823580.5252863361864237No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTG1798430.5180418219040295No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACGGCTACCA1705580.49129616977200924No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1585290.45664636368734884No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAA1353340.38983264250240446No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGG1288490.37115245358736393No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCA1282690.36948175049241816No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCAA1190420.3429031686698925No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAAGGCCTACC1140190.32843430376314636No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCGCTACCAA1092890.31480943197160566No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTAGGAACTGG1064520.3066373894192587No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGGT1037610.2988859031632257No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTG1025030.2952622057607398No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTAGGAACTGG1007730.29027890170167736No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCGTACCAA974100.2805917042735692No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTTGCAACTG887430.2556262150944395No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGG829990.23908049340932114No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGGGAACCGGTACCAA815600.23493542142030907No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGACCGAACCCCTACC751590.21649719640177795No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAA732750.2110702918657816No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCTCCGATCCCCTAC609520.17557361214333836No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACGCCTACCA575050.16564445081872084No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAACCCGTACCAA557610.16062081944357348No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGTCCGAAGGCCTACC440650.12693022737721824No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCAACCCCTACCAA418590.12057579457126921No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCA415940.11981245608823363No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATGCCCTAC385090.1109260439366685No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCTCCGAACCCCTAC378960.10916028359666544No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAAGCCCTACC372090.1071813645859279No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGATGCCCTAC364170.10489999070455365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG5399450.0144.799735
TACTGTA4198050.0144.766832
ATAACGC5412700.0144.719886
CGATAGG5008300.0144.685736
ACGATCG5004300.0144.649351
TAACAAT5073350.0144.580721
CGATTCC5433450.0144.530173
TCGCATC3299750.0144.44967
TAACGCA5423050.0144.424037
TCGATTC5443800.0144.363272
ATACAGG5943050.0144.353567
GATTCCT5443700.0144.333974
GATACAG5950150.0144.282466
TACAGGT5946300.0144.246618
ATACTGT4210550.0144.239641
CGATAAC5426300.0144.208834
TCGATAA5429850.0144.126573
ATAGGTA5064950.0144.111428
ATCGATT5452800.0144.082571
ACGCATT5430050.0144.033579
Produced by FastQC (version 0.11.5)