FastQCFastQC Report
Fri 18 Oct 2019
HW3KNAFXY_n01_PCR2_R3_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3KNAFXY_n01_PCR2_R3_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTGCAACTG97.377049180327869No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACGTACTGGT75.737704918032787No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAA64.918032786885246No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTTCCAACTG54.098360655737705No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG43.278688524590164No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGGAACTGG43.278688524590164No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCA43.278688524590164No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA43.278688524590164No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACTGG43.278688524590164No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACGCCTACC43.278688524590164No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC43.278688524590164No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACGCCTACCTACTGGT32.459016393442623No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGGAACTGG32.459016393442623No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC32.459016393442623No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC32.459016393442623No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGTACTGGT32.459016393442623No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAA21.639344262295082No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCA21.639344262295082No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATCCCCTAC21.639344262295082No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGG21.639344262295082No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACGGCTACCA21.639344262295082No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT21.639344262295082No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG21.639344262295082No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGG21.639344262295082No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGAACCCCTAC21.639344262295082No Hit
CTGTACCATGCTCATGCACACAAAAATACAGATAGCAGTCGGGGAGTGAA10.819672131147541No Hit
ATCTATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAA10.819672131147541No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAAGCCCTAGGAACTGG10.819672131147541No Hit
GCGAGGTCGCATCTTGTGGAAAGGACTAAACACCGATCCCCTTCCAACTG10.819672131147541No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTAGGAACTGG10.819672131147541No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTGCGAACCGCTACCA10.819672131147541No Hit
ATGCTGCTTCTGGTGTGGTAGATAAATAACAAGGAATACATAAACCTGTT10.819672131147541No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATGCCCTACGAACTGG10.819672131147541No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCCGTACCAA10.819672131147541No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAAGCCCTACCAACTTGT10.819672131147541No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAA10.819672131147541No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGACGCCATCC10.819672131147541No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTG10.819672131147541No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTTCCCCTTGCAACTG10.819672131147541No Hit
TAACAATGTCTTGTGGCAGGACGAAACACCGAACCCCTACCATGTGGTCG10.819672131147541No Hit
CGCTCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC10.819672131147541No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATGCCCTAC10.819672131147541No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCGCTACCA10.819672131147541No Hit
CGGGGATAAATTTTAGCAGATTTGTAGTCACAGAATGCGCCCGGAAAACG10.819672131147541No Hit
GGGTGGTGGCAGGTTGTGGAAAGGACGAGACACCGATCCCCTTGCCCCTC10.819672131147541No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC10.819672131147541No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG10.819672131147541No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTG10.819672131147541No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGAACCGGTACCAA10.819672131147541No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACGGCTACCA10.819672131147541No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC10.819672131147541No Hit
GATCGATAACGCATTTCTAGTGGAAAGGACGAAACTCCGATCCCCTACCA10.819672131147541No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAA10.819672131147541No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCA10.819672131147541No Hit
GGGTGGCGGCAGGTCGTCGTGAGGACGGGACGCCGCTCCGCTGGCCTCAC10.819672131147541No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAGAAACTCCGAACGCCTACC10.819672131147541No Hit
TATAGATATTCAAATAACCATGAAACAAAAGCTTAGCGTTTTTATAGGTA10.819672131147541No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGAACCCCTAC10.819672131147541No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTGGT10.819672131147541No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)