FastQCFastQC Report
Fri 18 Oct 2019
HW3KNAFXY_n01_PCR2_R2_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3KNAFXY_n01_PCR2_R2_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAAGACCGATCCCCTAC211.11111111111111No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGTACTGGT211.11111111111111No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTAGGAACTGG15.555555555555555No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCA15.555555555555555No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC15.555555555555555No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC15.555555555555555No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG15.555555555555555No Hit
TAACGATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGTACTGGT15.555555555555555No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACAGCGAACCCCAACCA15.555555555555555No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAA15.555555555555555No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTG15.555555555555555No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGATCCCCTAGGAACTGG15.555555555555555No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACGCCTACGTACTGGT15.555555555555555No Hit
ACGTTCGATAGGTAAGGTCTTGTGGAAAGGACGAATGACCGATCCCCTAC15.555555555555555No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAACCCCTACC15.555555555555555No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGAAGCCCTACC15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)