FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437212
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59300.17252354524539074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT44950.020.6461185
CGATCTG111600.020.0939335
CCGATCT269300.018.434614
TCCGATC284150.017.6775953
TTCCGAT287450.017.5469192
CGCCGTT11100.017.40603325
GATCTGG41100.017.2918386
GACCGTT10600.017.2498687
ACGGCCG10850.017.17087620
ACCGTTA10700.017.0886578
GGACCGT11950.017.0333426
CTTCCGA301000.017.021131
TACGGCC11650.015.9917619
CCGTTTA10900.015.82625927
GCCGTTT12450.015.51863226
GATCTTT32300.015.4874996
TGCACGA8800.015.28943442
TTAGGAC16300.015.2389263
TAGGACC14950.015.2304324
CCGTTAT12050.015.17469
Produced by FastQC (version 0.11.2)