FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29781
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335311.258856317786508No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGT150.002333002969.0000134
CATTAAA150.002333002969.0000123
TTTGGCG150.002333002969.0000126
GTCCGCC150.002333002969.0000131
TACTATA150.002333002969.000016
TCATTAA200.0072891551.75000422
TTTTGTG200.0072891551.75000435
TTTTTTA450.004551423730.66666639
AATTAGA1352.4835026E-723.069
GAATTAG1702.2748009E-618.26470668
ACGAATT2251.0048825E-716.86666768
CGAATTA2301.291155E-716.569
GACGAAT2759.798387E-713.867
CCGACGA3407.80301E-712.17647165
CGACGAA3154.5030483E-612.0476266
GCCGACG3802.2869972E-711.80263164
TGCCGAC3753.119793E-510.1263
CTGCCGA3955.5239707E-59.60759562
GCTGCCG4159.4842966E-59.14457861
CGCTGCC4703.6586716E-48.07446960
Produced by FastQC (version 0.11.2)