FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173342
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35850.16495332994070883No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT450.00462420630.6658331
CGATCTG39700.030.2430745
GATCTGG20850.030.1163646
CCGATCT99100.028.3037574
TTCCGAT100950.027.7508892
TCCGATC102400.027.3242423
CTTCCGA116350.024.6412011
ATCTGGG14650.022.8439567
CGATCTT43750.021.0557355
ACCGGTA2650.020.83105715
TCTTCCG76250.020.2257615
ATCTGGC11050.019.9827357
ATCTGCG7800.019.4623497
TCTGCGC4400.018.8189688
TGCGCGG2053.8275175E-818.51211410
GGTGTAG8600.018.05183269
GTGCTCT87000.017.9244731
CGGGAGA11400.017.85600715
CCGGTAA3500.017.74359716
GCTCTTC88600.017.600783
Produced by FastQC (version 0.11.2)